TRYPTOPHAN HYDROXYLASE; SH X-100UL

Analysis Note

ControlBrain tissuePC12 lysates

Application

Detect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.

Immunohistochemistry(peroxidase):
Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.

Optimal working dilutions must be determined by the end user.

General description

Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.

Immunogen

Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: AB1556

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified sheep polyclonal liquid in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 µg/mL BSA and 50% glycerol.

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 µg of PC12 lysates.

Specificity

Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species.

Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.

Storage and Stability

Stable for 6 months at -20°C to -80°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

Target description

55 kDa